FIGURE 2.

Examining PPARγ-2 and Runx2 mRNA using reverse transcription and qRT-PCR in fetal MSCs. (A) Dose-dependent enhancement of nonspecific siRNA on PPARγ-2, but not Runx2 expression. RNA samples were extracted from cells grown in basal cell culture medium (basal) or cells transfected with the nonspecific siRNA control 1(NS-siRNA 1) at indicated concentrations or without siRNA (Mock), followed by adipogenic induction for 14 d. (B,C) PPARγ-2 expression in cells with mock or NS-siRNA 1 transfection and adipogenic induction for 0, 7, and 14 d, respectively. Each treatment was established in duplicate and each sample was examined in duplicate. PPARγ-2 or Runx2 mRNA levels in cells with mock transfection at day 14 of adipogenic induction (B,C) were taken as 1.0. Relative PPARγ-2 or Runx2 mRNA levels for each treatment are presented as mean ± SD.