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. Author manuscript; available in PMC: 2008 Apr 1.
Published in final edited form as: Biochim Biophys Acta. 2006 Dec 24;1773(4):483–501. doi: 10.1016/j.bbamcr.2006.12.008

Fig. 4. Time course of CCK stimulation of PKD1 S916 and S744/748 phosphorylation in rat pancreatic acini.

Fig. 4

Rat pancreatic acini were treated with no additions or with 10 nM CCK for the indicated periods of time and then lysed. Upper 2 panels: membranes were analyzed using anti-pS916 PKD1 Ab (panel 1). To verify loading of equal amounts of protein, membranes were stripped and re-blotted with anti-PKD1 Ab (panel 2). Lower 2 panels: membranes were analyzed using anti-pS744/748 PKD Ab (panel 3). To verify loading of equal amounts of protein, membranes were stripped and re-blotted with anti-PKD1 Ab (panel 4). The bands were visualized using chemiluminescence and quantification of phosphorylation was assessed using scanning densitometry. The upper part shows a representative experiment. The values shown in the bottom part are the means ± S.E. of 4 independent experiments and are expressed as fold increases over the pretreatment level (exp/control).