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. Author manuscript; available in PMC: 2007 Jul 19.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2006 Dec;47(12):5218–5226. doi: 10.1167/iovs.06-0254

Figure 1.

Figure 1

Serum-induced expression changes of CTCF and PAX6. (A) Western blot analysis of CTCF protein expression. CTCF protein was identified in various cell types by Western blot analysis using a specific anti-CTCF antibody. β-Actin was used as an internal control for Western blot analysis. (B) Expressions of CTCF and Pax6 mRNAs, and PAX6 protein in Rb cells in response to serum stimulation. Rb cells were cultured in the normal condition, as introduced in methods and serum-starved 36 hours before 10% FBS stimulation for 6 hours. Total RNAs of these cells were harvested for Northern blot analysis of CTCF and Pax6 mRNAs and Western blot analysis were performed to detect cellular protein expression of CTCF and Pax6. Afterward, the same membranes as were used in Northern and Western blot analyses were stripped and rehybridized with GAPDH probe and β-actin antibody, respectively as the internal controls.