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Generation of cAMP in response to α-MSH (filled circle), ACTH(1–24) (filled square), MSH-A (open circle), MSH-B (filled triangle) and ACTH (1–31) (open square) for the lamprey MCa receptors expressed in HEK-293 EBNA cells. Untransfected cells showed no adenylate cyclase activity in response to ligands (data not shown). The cAMP assay was performed in duplicate and repeated two times with each ligand.
