Table 2.
Sequences involved in direct crosslinks with keratin chains
| Chain | Sequence | Keratin chains yield, pmol
|
|||
|---|---|---|---|---|---|
| 1 | 2e | 5/6 | 6 | ||
| Keratin chain | |||||
| Keratin 1 (Lys-73) | NLGGSKSISISVAR | ||||
| Keratin 2e (Lys-69) | GLGGTKSISISVA | ||||
| Keratin 5 (Lys-71) | NLGGSKRISISTR | ||||
| Keratin 6 (Lys-68) | GLGGSKRISI | ||||
| Crosslinked partner | |||||
| Cystatin α (Gln-45) | TQVVAGTNY | 25 | 35 | ||
| Elafin (Gln-2) | AQEPVKGPV | 40 | 80 | ||
| Envoplakin* (Gln-1970) | AQLLQDESSFEKDL | 235 | 25 | 600 | 40 |
| Envoplakin (Gln-1973) | AQLLQDESSFEKDL | 80 | 460 | 30 | |
| Involucrin (Gln-88/525) | HLEQQEGQLK | 120 | 5 | 110 | |
| Involucrin (Gln-288) | YLEQQEGQLK | 170 | 20 | 130 | 20 |
| Involucrin (Gln-328) | HLEQQEGQLEQL | 20 | 5 | 110 | 5 |
| Involucrin (Gln-436) | HLEEQEGQLK | 40 | |||
| Involucrin (Gln-445) | HLEQQQGQLEV | 90 | 15 | ||
| Involucrin (Gln-446) | HLEQQQGQLEV | 50 | 10 | ||
| Involucrin (Gln-455) | PEQQVGQPKNL | 40 | 65 | 10 | |
| Involucrin (Gln-456) | PEQQVGQPKNL | 10 | |||
| Loricrin (Gln-6) | QKKQPTPQPPV | 40 | 10 | ||
| Loricrin (Gln-153) | SGQAVQCQSY | 70 | 10 | ||
| Loricrin (Gln-215) | YVSSQQVTQTSCA | 570 | 105 | 65 | |
| Loricrin (Gln-216) | YVSSQQVTQTSCA | 360 | 20 | 20 | |
| Loricrin (Gln-219) | SSQQVTQTSCA | 180 | 20 | ||
| Loricrin (Gln-303) | CHQTQQKQA | 30 | |||
| Loricrin (Gln-305) | HQTQQKQA | 20 | 40 | ||
| Loricrin (Gln-308) | HQTQQKQTW | 10 | 10 | ||
| SPR1 (Gln-5) | QQQKQPC | 10 | |||
| SPR1 or 3 (Gln-19) | QQQQV | 10 | |||
| SPR1 or 3 (Gln-83) | QQKTKQK | 90 | 10 | ||
| SPR1 or 3 (Gln-87) | QQKTKQK | 170 | 15 | ||
| SPR3 (Gln-5) | QQKQTF | 10 | |||
| SPR2 (Gln-6) | QQQQCKQPCQPPPV | 5 | |||
| SPR2 (Gln-9) | QQQQCKQPC | 5 | |||
Single-letter code is used for amino acids. Residues involved in crosslinks are shown in bold.
*
Note that three envoplakin variants have been described (15), but the data are combined here because the same Gln residues were used.