Figure 9.

Isolation and characterization of LECs from lymphangiomas. LECs from lymphangioma explants (patient-B) isolated by CD31 microbead sorting shown by phase contrast microscopy. The culture was contaminated with stromal cells (a). Isolation of LECs with CD31 micro beads. Indicated are cell numbers after each step.(b). Cells are further cultivated and displayed the typical cobblestone morphology (c). d) Same specimen as in c) stained for CD31 and Prox1. Magnification: × 40 in (a) and × 100 in (c) and (d).