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Selected query species. The three query species that had completed genome projects and complete global knockout data available
| Query Species | Domain | Taxonomy ID | Number of Proteins from NCBI RefSeq | Essential Genes that could be mapped to a NCBI RefSeq ID: |
| Bacillus subtilis (strain 168) | Bacteria | 224308 | 4105 | 271/271 |
| Escherichia coli (strain K12) | Bacteria | 83333 | 4237 | 299/303 |
| Saccharomyces cerevisiae | Eukaryote | 4932 | 5872 | 1050/1105 |
