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Mutually exclusive binding of YY1 and VDR/RXR heterodimers to the OC-VDRE. GST-YY1 and GST-RXRα were affinity-purified on glutathione-Sepharose beads (14). DNA binding assays were carried out in the absence or presence of GST-YY1 (50 ng) and GST-RXRα (100 ng) in the presence of increasing amounts of hVDR. All DNA–protein binding reactions were performed in the presence of 10−8 M 1,25(OH)2D3 for 30 min.
