Figure 1. Effects of O₂^•−, SOD1 and catalase on Cn activity in brain cytosol.

Rat brain cytosol was exposed to O₂^•−-generating system [25 μM xanthine (X) and 0.01 unit/ml XO], 0.67 μM SOD1, or both, in the absence or presence of 130 units/ml catalase. Cn activity was measured following pre-incubation with the various agents for 25 min at 30 °C. Control samples pre-incubated with buffer only. Results are means±S.E.M. Cn specific activities from duplicate determinations from three experiments. ***P<0.001.