Figure 4. Effect of various anions on the NADH- and NADPH-dependent activity of human BVR-A.
The enzyme (5 μg) was assayed using 700 μM NADH as the cofactor and 10 μM biliverdin as the substrate at pH 7.0 in sodium phosphate (○) and sodium pyrophosphate (□). Also shown are the results when human BVR-A was assayed using 100 μM NADPH and 10 μM biliverdin as substrate in sodium phosphate (●), pH 7.0, at the indicated concentrations.
