Figure 6. Apaf-1 is not necessary for receptor-mediated apoptosis of Jurkat cells.
(A) Wild-type (WT), control-vector-transfected (pSUP) and Apaf-1-deficient cells (106/ml) were cultured with agonistic anti-Fas antibody (50 ng/ml) for 6 h and processed for cell death determination by flow cytometry. Quadrants are defined as: live (lower left), early apoptotic (lower right), late apoptotic (upper right) and necrotic (upper left). Numbers refer to the percentage of cells in each quadrant. (B) Duplicate aliquots of cells were harvested and lysed for Western blotting.