Figure 4. Time course for His₆–WbpI-catalysed reactions after incubation at 37 °C.

All reactions contained 20 mM sodium phosphate (pH 6.0), 0.17 mM NADP⁺, 1 mM UDP-α-D-GlcNAc3NAcA and 0.06 mg/ml of His₆–WbpI. Each data point represents the mean±S.D. of the mean determined from two experiments performed in duplicate.