Figure 2.

Inhibition of cell-free translation. (A) Relative β-galactosidase activity after coupled transcription/translation in the presence of control PNAs 997 (⧫), 1197 (•), 1440 (▪); antiribosomal PNAs 647 (▴) and 1143 (▾) and the antibiotic tetracycline (○). (B) Transcription (broken lines) and translation (solid lines) in the cell-free system were assayed by measuring ³²P-UTP and [³⁵S]methionine incorporation in independent reactions in the presence of the antiribosomal PNAs. (C) Translation of a synthetic mRNA, assayed by measuring [³⁵S]methionine incorporation in the presence of control and antiribosomal PNAs. The values represent the average of duplicate measurements with the SD shown as error bars.