Figure 1.

SC35 functionally substitutes for U2AF⁶⁵ in the reconstitution of pre-mRNA splicing in U2AF-depleted extracts. (A) Splicing of PIPβG pre-mRNA in: mock depleted extract (lane 1); U2AF-depleted extract (lane 2); and U2AF⁶⁵-reconstituted (lane 3) and SC35-reconstituted (lane 4) U2AF-depleted extract. (B) Western analysis with anti-U2AF⁶⁵ antibody of mock depleted (lane 1) and U2AF-depleted (lane 2) extract. (C) Western analysis of (Left) crude lysate of SC35 overexpression with α-p50 (anti-Drosophila U2AF⁵⁰ antibody) and anti-SC35 antibody (mAb104) and (Right) purified SC35 with α-p50 and anti-SC35 antibody (mAb104).