Figure 1.

Schematic representation of the Man/S4GGnM-Fc chimera and the deletion mutants examined for their ability to bind Man-BSA and S4GGnM-BSA. The wild-type chimera (Wt) consists of a Cys-Rich domain (Cys-Rich Domain), a fibronectin (FN) type II repeat, eight CRDs, and the Fc region of human IgG1 (IgG-Fc), which has replaced the transmembrane and cytosolic domains of the native receptor (4). Deletion mutants of the receptor were prepared by inverse PCR (9). Regions shown in black were deleted. The ability to bind (+) or not bind (−) Man-BSA and S4GGnM-BSA is indicated for each mutant based on the results shown in Fig. 3. The EMBL accession number for the murine Man-R is Z11974.