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Overexpression of the dxs gene product in E. coli. (A) E. coli XL1-Blue cells harboring plasmid pTAC-ORF2 or pTACTAC were induced with isopropyl β-d-thiogalactoside, and samples were withdrawn at the indicated times. Proteins were analyzed by SDS/PAGE and Coomassie blue staining. The position of molecular mass markers is indicated on the left.
