Figure 3.

Lack of degradation of actin during apoptosis of BL30A cells induced by a variety of agents and serum withdrawal. (A) Effect of different agents and serum withdrawal on the cleavage of DNA-PKcs. Cells were treated with γ-irradiation (20 Gy) and incubated for 8 h; EGTA (5 mM) for 8 h; EDTA (10 mM) for 6 h; or serum was withdrawn for 24 h. In all cases, ≈40-60% of the cells were undergoing apoptosis. (B) Lack of degradation of actin after treatment of BL30A cells with the same agents and serum withdrawal. A polyclonal antibody against the C-terminal 11 amino acids of actin was used for the immunoblot.