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. Author manuscript; available in PMC: 2007 Jul 20.
Published in final edited form as: Mol Cell. 2007 Apr 27;26(2):217–229. doi: 10.1016/j.molcel.2007.04.007

Table 1.

Yeast Strains and Plasmids Used in This Study

Strain or Plasmid Description
Strains
 NOY388 MATa ade2-1 ura3-1 trp1-1 leu2-3,112 his3-11 can1-100
 NOY975 MATa ade2-1 ura3-1 trp1-1 leu2-3,112 his3-11 can1-100 rpa135Δ::LEU2 carrying pNOY199
 NOY1075 MATa ade2-1 ura3-1 trp1-1 leu2-3,112 his3-11 can1-100 rrn3(S213P) (see Claypool et al. [2004])
 NOY2169 MATa ade2-1 ura3-1 trp1-1 leu2-3,112 his3-11 can1-100 rpa135Δ::LEU2 carrying pNOY742
 NOY2171 MATa ade2-1 ura3-1 trp1-1 leu2-3,112 his3-11 can1-100 rpa135Δ::LEU2 carrying pNOY743
 NOY2172 MATa ade2-1 ura3-1 trp1-1 leu2-3,112 his3-11 can1-100 rpa135(D784G)
 NOY2173 MATa ade2-1 ura3-1 trp1-1 leu2-3,112 his3-11 can1-100 rpa135Δ::LEU2 carrying pNOY744
 NOY2174 MATa ade2-1 ura3-1 trp1-1 leu2-3,112 his3-11 can1-100 rpa135Δ::LEU2 carrying pNOY745
 NOY2175 MATa ade2-1 ura3-1 trp1-1 leu2-3,112 his3-11 can1-100 rrp6Δ::HIS3
 NOY2176 MATa ade2-1 ura3-1 trp1-1 leu2-3,112 his3-11 can1-100 rpa135(Δ784G) rrp6Δ::HIS3
Plasmids
 pNOY199 High copy number plasmid carrying GAL7-35S rDNA, 2 mm, TRP1
 pRS316 pBluescript, CEN6, ARSH4, URA3 (see Sikorski and Hieter [1989])
 pNOY742 pRS316 (pBluescript, CEN6, ARSH4, URA3) derivative containing RPA135
 pNOY743 pRS316 (pBluescript, CEN6, ARSH4, URA3) derivative containing rpa135(D784G)
 pNOY744 pRS316 (pBluescript, CEN6, ARSH4, URA3) derivative containing His6-(HA)3-RPA135
 pNOY745 pRS316 (pBluescript, CEN6, ARSH4, URA3) derivative containing His6-(HA)3-rpa135(D784G)
 pNOY746 Derivative of high copy number plasmid pNOY373 (Wai et al., 2000) containing rDNA with promoter, but with C mutated to G between +1 and +56 relative to transcription start site