Skip to main content
. 1998 Mar 3;95(5):2140–2145. doi: 10.1073/pnas.95.5.2140

Figure 2.

Figure 2

Sucrose gradient centrifugation of native, dissociated, and reassociated coatomer. Native coatomer was treated with the concentrations of DMMA indicated. To reassociate dissociated coatomer, the modified Lys ɛ-amino groups were regenerated by dialysis against 100 mM KCl/50 mM Hepes⋅KOH, pH 6.7/1 mM DTT (reassociation buffer). Samples of native, dissociated, and reassociated coatomer were separated on sucrose gradients and analyzed by SDS/PAGE on 7.5–16.5% gels, Western blotting with antibodies against all COPs. (A) Sedimentation of native coatomer. (B–D) Sedimentation of coatomer treated with the indicated concentrations of DMMA. (E) Sedimentation of DMMA-treated coatomer (1.5 mM) after dialysis against reassociation buffer. Lanes: 1–20, fractions from sucrose gradients collected from bottom to top; C, isolated coatomer as a standard.