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Isolation of a β/δ-COP dimer. Isolated coatomer was modified with 1.5 mM DMMA and chromatographed on a Mono Q anion-exchange column. Proteins were eluted with a salt gradient. The resulting fractions were analyzed for COPs by SDS/PAGE on 7.5–16.5% gradient gels and Western blotting with antibodies for all COPs. Fractions 13–15 containing β/δ-COP dimer were used for Golgi binding assay. ∗, Cross-reactivity with anti-ɛ-COP serum.
