Table 1.
Rate constants for ribozyme 13/40 cleavage at various triplets
| Cleavable triplet | Single-turnover
|
||
|---|---|---|---|
| Multiple-turnover
|
k′cat, min−1 | ||
| kcat, min−1 | Km, nM | ||
| A16.2U16.1G17↓A1.1 | 0.14 | 30.33 | 0.88 |
| A16.2U16.1A17↓A1.1 | 0.10 | 34.09 | 0.38 |
| A16.2U16.1U17↓A1.1 | NA | NA | 0.007 |
| A16.2U16.1C17↓A1.1 | NA | NA | NA |
| A16.2A16.1G17↓A1.1 | 0.19 | 108.7 | 0.54 |
| A16.2U16.1G17↓U1.1 | 0.08 | 80.06 | 0.40 |
| A16.2U16.1G17↓G1.1 | 0.46 | 172.05 | 0.68 |
| U16.2U16.1G17↓A1.1 | ND | ND | 0.83 |
| G16.2U16.1G17↓A1.1 | ND | ND | 1.19 |
The cleavage position is indicated by an arrow. Reaction conditions were as described in Materials and Methods. NA, no activity detectable; ND, not determined; deviations from the AUG-A sequence are underlined.