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. 1998 Mar 3;95(5):2163–2168. doi: 10.1073/pnas.95.5.2163

Figure 3.

Figure 3

SR protein-mediated exon/exon associations are enhancer-dependent. Shown is relative association of UP exon 5, DOWN exon 5, and HET exon with R17-exon columns in the presence and absence of SR proteins. The relative amount of UP exon 5 and DOWN exon 5 RNA added to R17-exon 6 (A) or R17-UP affinity columns (B) is shown (lanes 1 and 2, respectively). The signals from the UP exon 5 and DOWN exon 5 RNAs are shown from complexes assembled either in the absence (lanes 3 and 4, respectively) or presence (lanes 5 and 6, respectively) of SR proteins. A darker exposure of the control RNA is shown for the complexes assembled with R17-exon 6 (lanes 3–6). (C) The relative amount of UP exon 5 and HET exon RNA added to the R17-UP affinity columns is shown (lanes 1 and 2, respectively). The signals from the UP exon 5 and HET exon RNAs are shown from complexes assembled either in the absence (lanes 3 and 4, respectively) or presence (lanes 5 and 6, respectively) of SR proteins. In all experiments, one-third of each RNA-affinity complex was loaded on each lane. The relative migration of the exon RNAs and the internal control RNAs is indicated to the right of each figure by arrows and Cs, respectively.