Figure 1.

HPLC chromatogram of pepsin digests of E. coli-derived rhGIF. The column was eluted with a linear gradient of solution B from 5% to 25%, indicated by the broken line. Peptide peaks, determined by absorption at 215 nm, are numbered. cysteine-containing peptides are indicated by arrows. Some of the peptide peaks, such as PP-8, PP-10, and PP-11, contained two different peptides. Amino acid sequences of all peptides recovered from the column are shown below the chromatogram.