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Fractionation of EMTS-treated GIF and DTNB-treated GIF on a CM-5PW column. The starting buffer for the fractionation of EMTS-treated GIF (A) was 10 mM phosphate buffer (pH 6.5) containing 0.08 M NaCl, while that for DTNB-treated GIF (B) was the same buffer without NaCl. Proteins were eluted by a linear gradient of NaCl, indicated by a broken line. The arrow represents the elution time of untreated GIF applied to the same column.
