Figure 5.

Peptide map of a pepsin digest of a DTNB-derivative of rhGIF. The derivative isolated by chromatography on a CM-5PW column (see Fig. 4B) was digested with pepsin, and a peptide map was obtained under the same conditions as that in Fig. 1. Absorbance at 215 nm is shown by the solid line, and percentage of solution B for elution is given by the broken line. A Cys-60-containing peptide was recovered in the peak, which is indicated by an arrow. Amino acid sequence of the peptide was identical to that shown in Fig. 1 (PP-8). A Cys-57-containing peptide and a Cys-81-containing peptide were recovered in the same peak, indicated by an arrow. Amino acid sequences of these peptides were identical to those shown in Fig. 1 (PP-10).