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. 1997 Jan 7;94(1):213–218. doi: 10.1073/pnas.94.1.213

Figure 2.

Figure 2

Lack of CD8+ T cells in the spleen of BDC2.5/NOD.scid mice. Three-color flow cytometric analysis of splenocytes from negative littermate (NOD.scid/+; Left), TCR transgenic (BDC2.5/NOD.scid/+; Center), and TCR/NOD.scid (BDC2.5/NOD.scid; Right) mice was performed using anti-CD4, anti-CD8α, and anti-Vβ4 TCR mAb. (Top) Two-color CD4 versus CD8 dot plots. (Middle) Vβ4 TCR expression on gated CD4+CD8− (CD4+), CD4−CD8+ (CD8+) and CD4−CD8− (DN) subpopulations. (Bottom) Two-color dot plots, B220 versus sIgM confirms scid phenotype of B cells in bottom right panel.