Figure 4.
T cells from diabetic BDC2.5/NOD.scid mice efficiently transfer insulitis and diabetes to NOD.scid recipients. NOD.scid mice received 106 T cells from BDC2.5/NOD.scid mice by intravenous injection. Recipients were then followed for evidence of insulitis and diabetes. (a) Insulitis was assessed from multiple hematoxylin and eosin sections of pancreas. Insulitis was scored as peri- and mild insulitis (open bars), moderate (hatched bars), or severe (filled bars). Insulitis is represented as the composite of 40–100 islets per animal and 3–6 mice per time point. (b) Cumulative incidence of diabetes in 16 NOD.scid recipients of 106 T cells from diabetic BDC2.5/NOD.scid mice. Diabetes was assessed by measurement of blood glucose using a glucometer. Mice were considered diabetic after two consecutive readings ≥250 mg/dl (13.75 mM). Diabetes was dated to the first sequential readings.