Figure 6.

Presence of apoptotic β cells correlates with the level of insulitis. Pancreata from 31 normoglycemic NOD.scid recipients of T cells from diabetic BDC2.5/NOD.scid mice were analyzed for insulitis and apoptosis. Multiple sections from five distinct layers from each pancreas were stained by TUNEL and anti-insulin reagents and with hematoxylin. Islets were scored independently for insulitis and apoptosis. On average, 100 islets were scored per mouse. A second-order relationship (solid curve) was found using the graphpad software (r = 0.8963).