Table 1.

Equilibrium dissociation constants (K_d) for EcoRI–DNA complexes

Osmotic pressure, atm	Canonical site GAATTC, pM	Alternate site TAATTC, nM	Nonspecific site TAGACG, μM
0	400  (±100)	840  (±430)	8  (±4)
10	100  (±30)	300  (±150)	5  (±3)
20	60  (±40)	40  (±20)	3  (±2)
30	20  (±10)	9  (±6)	1.1  (±0.8)
40	4  (±3)	1.0  (±0.4)	0.9  (±0.5)
50	2  (±1)	0.4  (±0.2)	0.4  (±0.2)

K_d values are the average of at least five independent determinations from experiments like those shown in Fig. 1. Errors indicate 1 SD.