Knockdown of br expression in the adult female mosquito. dsRNAs corresponding to individual BR isoforms were injected into thoraces of female mosquitoes as described in Section 2. mRNA expression of Z1 (A), Z2 (B), Z3 (C) and Z4 (D) was measured by means of real-time PCR at the indicated time points after blood feeding. Each sample was analyzed in triplicate and normalized to the internal control, β-actin mRNA. WT, uninjected A. aegypti Rockefeller/UGAL strain; Mal RNAi, injected with double-stranded RNA complementary to bacterial malE; Z1-Z4, injected with dsRNA corresponding to the isoform-specific regions of the mosquito BR isoforms.