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. Author manuscript; available in PMC: 2007 Jul 20.
Published in final edited form as: Mol Cell Endocrinol. 2007 Jan 20;267(1-2):97–105. doi: 10.1016/j.mce.2007.01.006

Fig. 2.

Fig. 2

The BR isoforms are required for proper Vg expression in the fat body after blood ingestion. dsRNAs corresponding to individual (A) or a combination of two (B) BR isoforms were injected into the thoraces of newly emerged female mosquitoes. The transcripts of Vg in female mosquitoes were then measured using real-time RT-PCR at the indicated time after blood feeding and were normalized to β-actin expression. Please note the different Y-axis scale used in Panel B for animals at 48 h PBM. WT, uninjected A. aegypti Rockefeller/UGAL strain; Mal, injected with double-stranded RNA complementary to bacterial malE; Z1, Z2, Z3 and Z4, injected with BR isoform-specific dsRNA; Z1/Z4, injected with a mixture of dsRNA for Z1 and Z4. Representative data (mean ± S.E.M.) from at least three independent experiments are shown.