Fig. 3.

RNAi-mediated reduction in mosquito BR leads to abnormal expression of the USP gene. dsRNAs corresponding to individual BR isoforms were injected into thoraces of newly emerged female mosquitoes. The usp-a (A) and usp-b (B) transcripts in the fat body were then measured using real-time RT-PCR at the indicated time after blood feeding as described in Section 2. WT, uninjected A. aegypti Rockefeller/UGAL strain; Mal, injected with double-stranded RNA complementary to bacterial malE; Z1, Z2, Z3 and Z4, injected with BR isoform-specific dsRNA.