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. Author manuscript; available in PMC: 2007 Jul 20.
Published in final edited form as: Mol Cell Endocrinol. 2007 Jan 20;267(1-2):97–105. doi: 10.1016/j.mce.2007.01.006

Fig. 4.

Fig. 4

The BR binding sites on the Vg promoter are essential for transcriptional regulation by the BR isoforms. Drosophila L57 cells were transfected with 100 ng phRL-CMV (Renilla luciferase control vector), 100 ng of different reporter plasmids and 100 ng expression vectors for AaEcR-B, AaUSP-B, AaBR-Z1 (A) and AaBR-Z2 (B). A schematic diagram of the reporter constructs is depicted at the bottom of each panel. Transfections with increasing amounts of AaBR-Z1 are indicated as “+” (100 ng) and “++” (300 ng). After transfection, cells were cultured in medium with or without 1 × 10-6 M of 20E. Data represent the ratios of firefly luciferase to Renilla luciferase activity (relative luciferase activity). Values are means of triplicate independent transfection experiments, with error bars representing the S.D. of the mean.