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. Author manuscript; available in PMC: 2007 Jul 20.
Published in final edited form as: Mol Cell Endocrinol. 2007 Jan 20;267(1-2):97–105. doi: 10.1016/j.mce.2007.01.006

Fig. 6.

Fig. 6

BR is physically associated with EcR/USP in vitro. L57 cells were co-transfected with expression vectors for AaEcR-B, AaUSP-B and individual BR-V5 fusion proteins. Cell extracts were incubated with antibodies against EcR, and the precipitated proteins were subjected to Western-blot analysis with V5 antibody. At the bottom, AaEcR-B, AaUSP-B and AaBR proteins in the inputs were examined by mean of immunoblotting using antibodies against AaEcR, DmUSP and V5 tag, respectively. When the experiment was conducted using 20E, 1 × 10-6 M 20E was added into the binding and washing buffers.