FIGURE 5.

Recovery of sensitivity of Isc_eq to shaking after treatment with chloral hydrate. Monolayers were treated with 4 mM chloral hydrate for 4 days. After removal of chloral hydrate, some monolayers were placed on the orbital shaker at a frequency of 0.9 Hz and the others served as unshaken controls. Relative current is defined as the ratio of Isc_eq from shaken monolayers to those from unshaken monolayers. Amiloride-sensitive Isc_eq of the unshaken control monolayers recovers to the prechloral hydrate state within 24 h. The response of Isc_eq to shaking recovers with a time constant of ∼100 h. The absolute value for the control short-circuit current I_sc was ∼10 μA/cm², with a standard deviation of ∼1 μA/cm². The resistance of the shaken and unshaken monolayers averaged 1.5 ± 0.6 kΩ/cm² and 1.2 ± 0.4 kΩ/cm², respectively. The number of unshaken monolayers = 5, the number of shaken monolayers = 10. Asterisks indicate a p < 0.01 compared to unshaken monolayers.