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. 2007 Apr 27;93(4):1215–1223. doi: 10.1529/biophysj.106.100511

FIGURE 2.

FIGURE 2

Fluorescent images are shown for the slide after separation of the stamp. (A) Image of the Cy3 fluorescent signal of a spot after separation. (B) The same spot after binding of fluorescent-streptavidin to the free biotin. Dark squares correspond to the contact area where the balances have ruptured. Cy3START intensity and AF647START intensity have been derived from ROI-1 and ROI-3, respectively. Cy3REM intensity and AF647REM intensity have been derived from ROI-3 and ROI-4 and correspond to the grid around one contact area.