TABLE 1.

Four molecular force balances are depicted each comprising an amino-oligo (top), a Cy3-oligo (middle), and a biotin-oligo (bottom)

Dir.	Duplex	No.	Sequence
3′-5′	30PM	#66	NH2-20t-CTGCAGGAATTCGATATCAAGCTTATCGAT
5′-3′		#74	GACGTCCTTAAGCTATAGTTCGAATAGCTAc-17t-cATCGATAAGCTTGATATCGAATTCCTGCAGttttt-Cy3
3′-5′	30PM	#62	TAGCTATTCGAACTATAGCTTAAGGACGTC-20*t-Biotin
3′-5′	30GG	#66	NH2-20t-CTGCAGGAATTCGATATCAAGCTTATCGAT
5′-3′		#75	GACGTCCTTAAGGTATAGTTCGAATAGCTAc-17t-cATCGATAAGCTTGATATCGAATTCCTGCAGttttt-Cy3
3′-5′	30PM	#62	TAGCTATTCGAACTATAGCTTAAGGACGTC-20*t-Biotin
3′-5′	30CC	#66	NH2-20t-CTGCAGGAATTCGATATCAAGCTTATCGAT
5′-3′		#80	GACGTCCTTAACCTATAGTTCGAATAGCTAc-17t-cATCGATAAGCTTGATATCGAATTCCTGCAGttttt-Cy3
3′-5′	30PM	#62	TAGCTATTCGAACTATAGCTTAAGGACGTC-20*t-Biotin
3′-5′	29CC	#66	NH2-20t-CTGCAGGAATTCGATATCAAGCTTATCGAT
5′-3′		#86	_ACGTCCTTAACCTATAGTTCGAATAGCTAc-17t-cATCGATAAGCTTGATATCGAATTCCTGCAGttttt-Cy3
3′-5′	30PM	#62	TAGCTATTCGAACTATAGCTTAAGGACGTC-20*t-Biotin

Amino- and biotin-oligos are identical in all balances. Mutations are only introduced by single nucleotide exchanges (30GG, 30CC, 29CC) and a single nucleotide deletion (29CC) in the Cy3-oligonucleotides (underlined bases).