Figure 2.

Expression of mediators for cell cycle progression, survival and apoptosis in HEY and OVHS1 cell lysates obtained from cells growing as monolayer or spheroids over 4 days. Cell lysates were prepared and the expression of (A) cyclin D2, (B) P-Akt, T-Akt and (C) caspase-3 was determined by Western blotting as described in the Materials and Methods. Membranes from P-Akt immunostaining were stripped and re-probed for the expression of total Akt. (D) Total protein loading was determined by probing the membranes for β-actin. The experiment is representative of three different experiments with similar results.