Figure 8.
Effect of blocking α2, β1 subunits and α2β1 integrin on (A) disaggregation of 4 day spheroids on CI, and (B) MMP2/9 activation in HEY spheroids grown on 0.5% agarose for 2 days. To determine the neutralizing effect of antibodies spheroids were treated with the antibodies (20 μg/500μl) for 30 min before plating on CI for disaggregating assay. For gelatin zymography cells (106/ml) were treated with the antibodies (20 μg/ml) before seeding on 0.5% agarose in SFM fror 48 h. The experiment was repeated on OVHS1 cells with similar results.