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Constructs used in this study. On the left are schematics of the different constructs; on the right are their sequences in the final region of the homeodomain (HD) and directly downstream of it, with different colors shading the parental sequences of XOTX2 (red), XOTX5b (yellow) and OTD (blue). Lines are introduced for sequence alignment. The divergent region responsible for the different retinal activities of XOTX2 and XOTX5b (RS box) is shown in the blue box.
