Figure 6.

XOTX2 and XOTX5b differentially synergize with XNRL to activate the rhodopsin promoter and differentially interact in vitro with XNRL. (a) Results of rhodopsin promoter cell transfection assays with several Xotx/otd constructs (with or without Xnrl). Error bars indicate the standard error of the mean. (b) GST-pull down assays compare the interaction of MYC-XOTX/OTD fusion proteins to GST-XNRL or GST alone. The band indicated by an asterisk corresponds to a higher molecular weight (55 kDa) than the one expected for XOTX proteins (41 kDa) and may result from post-translational modification; this needs further investigation. (c) Results of two of these experiments, analyzed by Image J, were statistically processed; columns show the ratio of the retained MYC-tagged proteins relative to their respective input, normalized with respect to the MYC-XOTX2 retained/input ratio. Error bars indicate the standard deviation. The p-value was calculated by bilateral Student's t-test. Asterisks in histograms show statistically significant differences only for more relevant comparisons (*p < 0.05, **p <0.01). (see Additional files 12 and 13 for the full data set).