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Quantitative strand-specific RT-PCR analysis of SAS pair expression in different organs. 1: liver, 2: lung, 3: spleen, 4: kidney, 5: thymus. cDNAs were analyzed using SYBR real-time qPCR. Black bars represent sense transcripts, grey bars the corresponding antisense transcripts. SYBR qPCR for β-actin was used for normalization to control for variations in RNA input. The unit is CT(H2O)-(CT(transcript)-CT(β-actin)). Data shown are mean values of duplicate determinations.
