Table 3.
Results of an enrichment analysis on 145 genes that show significant differences in expression between the four different species of Apis
| GO ID | Term | Level | No. in experiment | Expected frequency | No. significantly regulated | Observed frequency | p value | Fly orthologs in 147 genes |
| GO:0009607 | response to biotic stimulus | 4 | 93 | 0.0513 | 20 | 0.1869 | 1.15E-07 | Tctp, Hsp70Aa, e, CG7966, Hsc70Cb, CG32687, Hop, Tsp96F, Hsp83, CG10178, CG5001, Ugt86Dd, Hsc70-4, PGRP-SC2, Gp93, dsd, Tsf1, PebIII, 18w, l(2)efl |
| GO:0006952 | defense response | 5 | 89 | 0.0491 | 19 | 0.1776 | 2.79E-07 | Tctp, Hsp70Aa, e, CG7966, Hsc70Cb, CG32687, Hop, Tsp96F, Hsp83, CG10178, CG5001, Ugt86Dd, Hsc70-4, PGRP-SC2, Gp93, dsd, Tsf1, 18w, l(2)efl |
| GO:0009408 | response to heat | 6, 5 | 12 | 0.0066 | 5 | 0.0467 | 0.0003452 | Hsp70Aa, Hsp83, CG5001, Hsc70-4, l(2)efl |
| GO:0006457 | protein folding | 7 | 45 | 0.0248 | 9 | 0.0841 | 0.0007291 | Hsp70Aa, Hsc70Cb, Hop, Hsp83, FKBP59, CG5001, Hsc70-4, Gp93, l(2)efl |
| GO:0006950 | response to stress | 4 | 65 | 0.0359 | 11 | 0.1028 | 0.0008152 | Hsp70Aa, Hsc70Cb, Hop, Tsp96F, Hsp83, CG5001, Hsc70-4, Gp93, PebIII, 18w, l(2)efl |
| GO:0042417 | dopamine metabolism | 9, 8, 6 | 2 | 0.0011 | 2 | 0.0187 | 0.0034525 | e, Dat |
| GO:0043473 | pigmentation | 2 | 8 | 0.0044 | 3 | 0.028 | 0.0083358 | e, b, Dat |
| GO:0048066 | pigmentation during development | 3 | 8 | 0.0044 | 3 | 0.028 | 0.0083358 | e, b, Dat |
| GO:0005975 | carbohydrate metabolism | 5 | 113 | 0.0623 | 12 | 0.1121 | 0.0172185 | Pglym78, CG6439, CG10178, Mdh, l(2)01810, Sulf1, Ugt86Dd, CG1271, PGRP-SC2, Sodh-2, CG14621, α-Man-IIb |
| GO:0042752 | regulation of circadian rhythm | 4, 5 | 4 | 0.0022 | 2 | 0.0187 | 0.0183819 | Hsp83, Dat |
| GO:0046916 | transition metal ion homeostasis | 8 | 4 | 0.0022 | 2 | 0.0187 | 0.0183819 | Tsf1, CG4349 |
| GO:0006584 | catecholamine metabolism | 7, 8 | 4 | 0.0022 | 2 | 0.0187 | 0.0183819 | e, Dat |
| GO:0006519 | amino acid and derivative metabolism | 5 | 67 | 0.037 | 8 | 0.0748 | 0.0252803 | e, CG1732, b, CG6028, CG6439, Eaat2, CG8745, Dat |
| GO:0009628 | response to abiotic stimulus | 4 | 80 | 0.0441 | 9 | 0.0841 | 0.0254136 | Hsp70Aa, wun, Rh6, Hsp83, CG10178, CG5001, Ugt86Dd, Hsc70-4, l(2)efl |
| GO:0007530 | sex determination | 3 | 12 | 0.0066 | 3 | 0.028 | 0.0258228 | fru, gro, CG3726 |
| GO:0009613 | response to pest, pathogen or parasite | 6, 5 | 12 | 0.0066 | 3 | 0.028 | 0.0258228 | Tsp96F, PebIII, 18w |
| GO:0018958 | phenol metabolism | 6 | 5 | 0.0028 | 2 | 0.0187 | 0.0288583 | e, Dat |
| GO:0006807 | nitrogen compound metabolism | 4 | 83 | 0.0458 | 9 | 0.0841 | 0.0302461 | e, CG1732, b, CG6028, CG6439, pyd3, Eaat2, CG8745, Dat |
| GO:0016614 | oxidoreductase activity, acting on CH-OH group of donors | 4 | 35 | 0.0197 | 7 | 0.066 | 0.0029256 | CG6439, CG10638, Mdh, CG9360, Sodh-2, antdh, CG10962 |
| GO:0008171 | O-methyltransferase activity | 6 | 2 | 0.0011 | 2 | 0.0189 | 0.0035187 | CG10527, Pcmt |
| GO:0005386 | carrier activity | 3 | 131 | 0.0736 | 15 | 0.1415 | 0.0052529 | CG32250, CG31547, CG1732, Cralbp, CG9317, Eaat2, l(3)neo18, Tsp5D, CG33310, l(2)01810, CG1271, Psa, Tsf1, PebIII, CG14621 |
| GO:0051082 | unfolded protein binding | 4 | 22 | 0.0124 | 5 | 0.0472 | 0.0066411 | Hop, Hsp83, CG5001, Hsc70-4, Gp93 |
| GO:0004263 | chymotrypsin activity | 7 | 3 | 0.0017 | 2 | 0.0189 | 0.0099384 | CG32130, CG4998 |
| GO:0015020 | Glucuronosyltransferase activity | 6 | 3 | 0.0017 | 2 | 0.0189 | 0.0099384 | CG10178, Ugt86Dd |
| GO:0008238 | exopeptidase activity | 5 | 17 | 0.0096 | 4 | 0.0377 | 0.0131342 | east, CG4678, Ance, Psa |
| GO:0015290 | electrochemical potential-driven transporter activity | 4 | 48 | 0.027 | 7 | 0.066 | 0.0149443 | CG31547, CG1732, CG9317, Eaat2, l(2)01810, Psa, CG14621 |
| GO:0015291 | porter activity | 5 | 48 | 0.027 | 7 | 0.066 | 0.0149443 | CG31547, CG1732, CG9317, Eaat2, l(2)01810, Psa, CG14621 |
| GO:0015293 | symporter activity | 6 | 18 | 0.0101 | 4 | 0.0377 | 0.0159093 | CG31547, CG1732, Eaat2, l(2)01810 |
| GO:0016831 | carboxy-lyase activity | 5 | 10 | 0.0056 | 3 | 0.0283 | 0.0162543 | b, CG6028, Mdh |
| GO:0008237 | metallopeptidase activity | 5 | 38 | 0.0214 | 6 | 0.0566 | 0.0165483 | Nep2, east, CG4678, Ance, Psa, S2P |
| GO:0008199 | ferric iron binding | 7 | 4 | 0.0022 | 2 | 0.0189 | 0.0187129 | Tsf1, CG4349 |
| GO:0016810 | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds | 4 | 19 | 0.0107 | 4 | 0.0377 | 0.0189845 | pyd3, Sirt2, gro, PGRP-SC2 |
| GO:0008509 | anion transporter activity | 4 | 31 | 0.0174 | 5 | 0.0472 | 0.0251189 | CG31547, Eaat2, Atet, l(2)01810, CG14621 |
| GO:0015296 | anion:cation symporter activity | 7, 5 | 12 | 0.0067 | 3 | 0.0283 | 0.0264295 | CG31547, Eaat2, l(2)01810 |
| GO:0015370 | solute:sodium symporter activity | 8, 6 | 12 | 0.0067 | 3 | 0.0283 | 0.0264295 | CG1732, Eaat2, l(2)01810 |
| GO:0019842 | vitamin binding | 3 | 5 | 0.0028 | 2 | 0.0189 | 0.0293608 | Cralbp, CG8745 |
| GO:0005283 | sodium:amino acid symporter activity | 7, 8, 9, 10, 5 | 5 | 0.0028 | 2 | 0.0189 | 0.0293608 | CG1732, Eaat2 |
| GO:0004295 | trypsin activity | 7 | 5 | 0.0028 | 2 | 0.0189 | 0.0293608 | CG32130, CG4998 |
| GO:0016830 | carbon-carbon lyase activity | 4 | 13 | 0.0073 | 3 | 0.0283 | 0.0323556 | b, CG6028, Mdh |
| GO:0008235 | metalloexopeptidase activity | 6 | 13 | 0.0073 | 3 | 0.0283 | 0.0323556 | east, CG4678, Psa |
| GO:0005279 | amino acid-polyamine transporter activity | 5, 6 | 14 | 0.0079 | 3 | 0.0283 | 0.0387781 | CG31547, CG1732, Eaat2 |
Statistical over-representation of GO categories was tested through a hypergeometric test followed by the Benjamini Hochberg FDR correction using the microarray analysis tool GOToolBox. See Table 6 for honey bee gene IDs