Table 4.
Functional clustering of 147 fly orthologs of genes showing significant differences in expression between honey bee species, based on Gene Ontology-defined biological processes
| Cluster | GO ID | GO category | Level | No. of genes | Fly orthologs | p value |
| 1 | GO:0007154 | cell communication | 3 | 7 | Sgt, CG11105, BM-40-SPARC, CG3876, inx3, Cad87A, Mnn1 | 0.02 |
| 2 | GO:0015031 | protein transport | 5,6 | 5 | Kif3C, CLIP-190, garz, CG31048, RhoBTB | 5.522e-06 |
| 3 | GO:0007275 | development | 2 | 7 | e, Dat, wun, Hsc70-4, bif, Hsp83, chic | 0.0148 |
| 4 | GO:0044237 | cellular metabolism | 4 | 17 | Tctp, CG10178, Ugt86Dd, Sulf1, CG10903, CG10638, Rpb8, pyd3, ACXD, Gycβ100Bb100B, CG6946, CG7564, Rsf1, fru, CG3726, Sirt2, gro | 0.009793 |
| 5 | GO:0050896 | response to stimulus | 3 | 7 | CG7966, dsd, Tsp96F, 18w, PebIII, Rh6, CG9265 | 0.005519 |
| 6 | GO:0006508 | proteolysis | 7 | 5 | Nep2, east, CG4998, CG4678, CG32130 | 0.002551 |
| 7 | GO:0019538 | protein metabolism | 5 | 12 | Rpn2, Nep2, east, CG4998, CG4678, CG32130, S2P, Pcmt, RpL6, Cralbp, Psa, FKBP59 | 2.287e-06 |
| 8 | GO:0006520 | amino acid metabolism | 6,7 | 5 | CG1732, Eaat2, CG6028, CG8745, b | 0.000116 |
Clustering was carried out in GOToolBox using the WPGMA algorithm with a Bonferroni correction for multiple testing. A minimum cluster size of 5 genes was applied. See Table 6 for honey bee gene IDs.