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. 2007 Jul 3;7:64. doi: 10.1186/1471-2180-7-64

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Copyright © 2007 Mundodi et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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T. foetus with surface AP33::HA displays enhanced level of adherence to immortalized MS-74 VECs. Transfected trichomonads with AP33::HA (bar labeled Tf-AP33) had higher levels of adherence compared to wt T. foetus (bar Tf), as before for heterologously-expressed AP65 [26]. The percent level of adherence was adjusted to that seen for T. vaginalis (Tv). Trichomonads expressing AP33::HA were pretreated with anti-AP33 antiserum (bar Tf-AP33 + antibody). The results are the average from four different experiments, and each experiment was carried out using quadruplicate samples.