Table 6.
PCR Primer sequences, length of PCR products and similarity with known genes
| Genes | primer sequences | length of PCR product (bp) | Similarities with homologous genes |
| Actin | S 5'-TCATTGGTATGGAAGCTGCTGAATC-3' A 5'-CCTGACTCATCATACTCGCCCTTCG-3' |
300 |
Oryza sativa actin mRNA, complete cds Identities = 279/330 (93%) |
| Actin | S 5'-GGAAGTACAGTGTCTGGATTGGAGGGT-3' A 5'-TTCAGAAGACCCAGACAACTCGCAAC-3' |
150 |
Oryza sativa actin mRNA, complete cds Identities = 140/150 (93%) |
| 14-3-3 | S 5'- CACTATGTCTGGGGTCG -3' A 5'- ATTTAGGACTTGCTGGCA -3' |
340 |
Triticum aestivum 14-3-3 protein Identities = 100% |
| RSG | S 5'-TGGGCTACCGGACTACGCCAAG-3' A 5'-CCT TGGAACTTGACCTGCCGCTT-3' |
367 |
Nicotiana tabacum mRNA for bZIP transcriptional activator RSG Identities = 67% |
| KAO | S 5'-ACAACTGCCTGGCCAAGATCACCAG-3' A 5'-GTGACAACTTTGACTCATCCGCGACAACAA-3 |
184 |
Hordeum vulgare KAO mRNA Identities = 166/184(90%) |
| KS | S 5' CCCCTGAACTTTCTGATGCTTGCATAT-3' A 5'-TTGAAGGACTGTACTTCTCAACCAATGCT-3' |
137 |
Hordeum vulgare KS-like gene Identities = 116/127 (91%) |
| TaGA2ox-1 | S 5'-TCGCTCCGCCTAAGCCACAG-3' A 5'-CTGCAACTCAAGCAGTCATCCCTC-3' |
104 |
Oryza sativa mRNA for OsGA2ox1 Identities = 73/104 (71%) |
| TaGA20ox-2 | S 5'-GCTGAGCCAGGGCGTGGAGAAG-3' A 5'-CCATGAAGGTGTCGCCGATGTTG-3' |
260 |
Oryza sativa gibberellin 20-oxidase 2 (Sd-1) gene Identities = 240/260 (92%) |
| TaGA20ox1D | S 5'-AGCACTACCGGGCGGACATGAA-3' A 5'-GCCATCCATCCATGCTTCTTCGTAC-3 |
260 | Wheat GA20-oxidase 1 gene homoeologous TaGA20ox1D Identities = 100% |
| TaGA3ox2-1 | S 5'-GTACATGGGCGTGCGCAAGAA G-3' A 5'-GCACGCATCCACCAGCATCATC-3' |
219 | Wheat GA3-oxidase 2 gene homoeologous TaGA3ox2-1 Identities = 100% |
| TaGA3ox2-2 | S 5'-GTACATGGGCGTGCGCAAGAAG-3' A 5'-CAGCTAAGCTACCAGCCCACCATG-3' |
258 | Wheat GA3-oxidase 2 gene homoeologous TaGA3ox2-2 Identities = 100% |
| TaGA3ox2-3 | S 5'-GTACATGGGCGTGCGCAAGAAG-3' A 5'-GCTAATCTAACAGCCCGCCACCAT-3' |
260 | Wheat GA3-oxidase 2 gene homoeologous TsGA3ox2-3 Identities = 100% |
| GAI | S 5'-GCACATTCCTGGACCGCTTCACC-3' A 5'-GCAGCCTTCCTTCTCCTCCACCTTG-3' |
422 |
Triticum aestivum Rht-1 gene Identities = 100% |
| TaGID1 | S 5'-CCACCATCGGCTTCTACCTGCTGTC-3' A 5'-GGCGAGCTCATCCACGACGAGAC-3' |
133 |
Oryza sativa mRNA for GID1 Identities = 120/133 (90%) |
| GAMYB | S 5'-CGTGAGAAGTTCAAGTTCCTCTGT-3' A 5'-AAGTTTTCAGGATGAGACGAAGTG-3' |
287 |
Triticum aestivum MYB3 gene Identities = 100% |
| GIP | S 5'-GGGACGCAGTACAAGAAGG-3' A 5'-GGAAACTGGGAGGGCAAT-3' |
300 |
Petunia hybrida mRNA for (gip5 gene) Identities = 64/69 (92%) |
| XET | S 5'-GCCCTTCGTCGCCTCCTAC-3' A 5'-CGGCACAACAACAACTAGTGGTAG-3' |
300 | Wheat mRNA for endo- xyloglucan transferase Identities = 100% |