Figure 9.

Full enhancer scanner analysis identifies less conserved sequences that are also part of conserved sequence blocks. The following Drosophila species were used to produce an EvoPrint of the Drosophila melanogaster 800 bp even-skipped stripe ^#1 enhancer [18]: D. melanogaster (reference sequence), D. simulans, D. sechellia, D. erecta, D. ananassae, D. persimilis, D. pseudoobscura, D. virilis, D. mojavensis and D. grimshawi. Drosophila yakuba was not included in the EvoPrint analysis due to lack of sequence co-linearity detected with EvoDifference prints. Invariant MCSs, shared by all species used to generate the EvoPrint, are identified with uppercase black-colored letters. A Full-enhancer scan of the enhancer with one of its 10 bp CSBs (blue highlight) revealed that it is repeated two additional times in the less conserved inter-block sequences (lowercase yellow highlighted sequences). Note that the underlined sequence in this CSB is the core DNA-binding sequence for the Tramtrack transcription factor.