Table 2.
List of genes identified by MGK as important for growth in the absence of aromatic amino acids
| Mutant library used | Genes | ||||
| Defined | trpAa (28.1) | pheAa (26.1) | epda (23.8) | trpEa (22.8) | aroEa (20.1) |
| tyrAa (17.5) | aroCa (14.8) | trpBa (13.9) | aroAa (13.3) | aroBa (11.9) | |
| tyrBa (12.2) | trpDa (7.4) | ygcL (6.6) | rcsF (6.3) | yddK (5.7) | |
| ygaF (5.4) | ynaJ (5.3) | pdxAa (5.2) | wzc (5.0) | surA (4.9) | |
| rpea (4.8) | ygdDa (4.7) | aroF (4.1) | yadN (4.0) | uvrY (4.0) | |
| yeeA (4.0) | ydhH (3.9) | yfhD (3.9) | yadK (3.8) | ompX (3.6) | |
| aroDa (3.5) | yfhM (3.5) | yedV (3.4) | ybeL (3.4) | trpCa (3.3) | |
| wcaA (3.3) | ydhX (3.2) | ||||
| Random | tyrBa (15.9) | epda (13.3) | trpAa (10.8) | aroEa (10.4) | tyrAa (7.8) |
| trpBa (3.9) | hepA (3.5) | tktAa (3.2) | |||
Values in parentheses are intensity ratios (normalized reference intensity value/normalized selection intensity value), and are the average of two independent, inversely labeled experiments. All mutants were individually tested. aMutants that exhibited a growth defect in the absence of aromatic amino acids. MGK, Monitoring of Gene Knockouts.