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. Author manuscript; available in PMC: 2007 Jul 20.
Published in final edited form as: J Invest Dermatol. 2007 Feb 1;127(6):1419–1429. doi: 10.1038/sj.jid.5700717

Figure 7. PAP-1 is not a contact sensitizer in vitro.

Figure 7

(a) MUTZ-3 and (b) THP-1 myeloid cell lines were cultured for 24 hours in the presence of vehicle (DMSO), the irritant SDS at 200 μM, the sensitizer dinitrochlorobenzene at 3.75 μM or PAP-1 at 10, 20, or 40 μM. CD86 expression was then measured by flow cytometry. Values are given as a stimulation index (SI), which is the ratio of CD86 expression of treated cells over CD86 expression of DMSO-treated cells (SI = (% of cells expressing CD86+ ×mean fluorescence intensity of CD86+ cells) of treated/(% of cells expressing CD86+ ×mean fluorescence intensity of CD86+ cells) of control). The values are the average±SEM of two independent experiments.