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Modulating the cell seeding density. (a) By varying the microwell-to-microwell spacing across the chip, we can pattern cells at multiple seeding densities on the same 60×15 mm TCPS dish. Cell patterning with cell-cell spacing of: (b) 200 μm, (c) 400 μm, and (d) 800 μm. Scale bar, 200 μm.